程永红¹，田莉虹^2*

（1.重庆市万州区疾病预防控制中心，重庆万州 404000；

2.重庆市江津区疾病预防控制中心，重庆江津 402260）

摘要：本文以常见的肉制品为研究对象，建立并优化了经过固相萃取小柱净化，结合LC-MS/MS测定肉制品中8种孕激素残留量的方法。样品经过酶解提取，之后经C18小柱净化后，采用液相色谱串联质谱对孕激素进行检测分析。结果表明，8种孕激素分离效果好，检出限（LOD）为0.01-0.05 μg/kg，相对标准偏差＜4.0%（n＝6），加标回收率达到76.8%-103.3%。表明该方法具有比较高的重现性和选择性，对于肉制品中孕激素的残留测定具有很好的应用价值。

关键词：孕激素；液相色谱串联质谱；肉制品

中图分类号：TS207.3   文献标识码：A   文章编号：1674-506X（2021）02-0161-0005

Determination of Progesterone Drug Residues in Food of Animal Origin by Liquid Chromatography Tandem Mass Spectrometry

CHENG Yong-hong¹, TIAN Li-hong^2*

（1.Chongqing Wanzhou District Center for Disease Control and Prevention, Wanzhou Chongqing 404000, China;

2.Chongqing Jiangjin District Center for Disease Control and Prevention, Jiangjin Chongqing 402260, China）

Abstract：This article takes common meat products as the research object，establishes and optimizes the method of solid phase extraction cartridge purification, combined with LC-MS/MS to determine the 8 kinds of progesterone residues in meat products. After the sample is enzymatically digested and extracted, purified by a C18 cartridge, the progesterone is detected and analyzed by liquid chromatography tandem mass spectrometry. The results showed that the separation effect of 8 progestogens was good, the limit of detection (LOD) was 0.01-0.05 μg/kg, the relative standard deviation was＜4.0% (n＝6), and the recovery rate of standard addition reached 76.8%-103.3% . This indicates that the method has relatively high reproducibility and selectivity, and has good application value for the determination of progesterone residues in meat products.

Keywords：progesterone; LC-MS-MS; meat products

doi：10.3969/j.issn.1674-506X.2021.02-026

收稿日期：2021-01-21

作者简介：程永红（1979-），男，本科，理化主管技师。研究方向：食品、饮用水、职业卫生理化检测。

*通信作者

引用格式:程永红,田莉虹.液相色谱串联质谱测定动物源性食品中孕激素类药物残留[J].食品与发酵科技,2021,57(2):161-165.